页岩气田含氧管道内硫酸盐还原菌生长特性

覃 敏, 廖柯熹, 白 杨, 廖呈龙, 韦孟佼

doi:10.16577/j.issn.1001-1560.2023.0005

材料保护 >

2023 , Vol. 56 >Issue 1: 30 - 34

DOI: https://doi.org/10.16577/j.issn.1001-1560.2023.0005

试验研究

页岩气田含氧管道内硫酸盐还原菌生长特性

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1. 西南石油大学石油与天然气工程学院油气储运研究所，四川成都 610500；2. 中国石油西南油气田公司，四川成都 610051； 3. 中国石油西南油气田输气管理处，四川成都 610213

覃敏(1997-)，主要从事石油与天然气领域研究，电话:18244239663，E-mail:1329314986＠qq.com

收稿日期: 2022-07-23

修回日期: 2022-08-20

录用日期: 2022-09-14

网络出版日期: 2023-07-25

基金资助

国家自然科学基金(52174062)资助；

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Growth Characteristics of Sulfate Reducing Bacteria in Oxygenated Pipeline of Shale Gas Field

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1. Institute of Oil and Gas Storage and Transportation， School of Petroleum and Natural Gas Engineering， Southwest Petroleum University，Chengdu 610500， China； 2. PetroChina Southwest Oil and Gas Field Company， Chengdu 610051， China；3. PetroChina Southwest Oil and Gas Field Transmission Management Office， Chengdu 610213， China

Received date: 2022-07-23

Revised date: 2022-08-20

Accepted date: 2022-09-14

Online published: 2023-07-25

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摘要

为了明确页岩气管线中硫酸盐还原菌的生长特性,采集某页岩气集气管线水样,并富集纯化其中的硫酸盐还原菌(SRB)。提取SRB的DNA并通过基因扩增技术(Polymerase Chain Reaction, PCR)对SRB群落特征进行了描述,定量测试了SRB在溶解氧环境下的生长特性。结果表明:页岩气集气管道内的SRB主要分为Bacteroidota、Firmicutes和Desulfobacterota。溶解氧的浓度对SRB的生长代谢有影响,在0~2.5 mg/L溶解氧下,SRB仍能大量生长繁殖;在4.5 mg/L及以上溶解氧下,SRB的生长代谢被完全抑制,说明在页岩气集气管道内,SRB能在低浓度O₂条件下生长繁殖。

关键词： 页岩气; 集气管道; 硫酸盐还原菌; 群落; 溶解氧

本文引用格式

覃敏, 廖柯熹, 白杨, 廖呈龙, 韦孟佼 . 页岩气田含氧管道内硫酸盐还原菌生长特性[J]. 材料保护, 2023 , 56(1) : 30 -34 . DOI: 10.16577/j.issn.1001-1560.2023.0005

Abstract

For clarifying the growth characteristics of sulfate reducing bacteria in shale gas pipelines, water samples were collected from a shale gas-gathering pipeline, and the sulfate reducing bacteria(SRB) in the samples were enriched and purified. The DNA of SRB was extracted, and then the gene amplification technology(Polymerase Chain Reaction, PCR) was utilized to describe the characteristics of SRB community. Furthermore, the growth characteristics of SRB in dissolved oxygen environment were quantitatively tested. Results showed that the SRB in shale gas gathering pipeline were mainly Bacteroidota, Firmicutes and Desulfobacterota. The concentration of dissolved oxygen had an impact on the growth and metabolism of SRB. Under a dissolved oxygen concentration of 0~2.5 mg/L, SRB could still grow and reproduce in large quantities. Furthermore, under a dissolved oxygen concentration of 4.5 mg/L and above, the growth and metabolism of SRB were completely inhibited, indicating that SRB were able to grow and reproduce under low O₂ concentration in the shale gas gathering pipeline.

Key words： shale gas; gas gathering pipeline; sulfate reducing bacteria; community; dissolved oxygen

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